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KMID : 0350519950480020363
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Journal of Catholic Medical College
1995 Volume.48 No. 2 p.363 ~ p.377
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The Effect of Colchicine on Alveolar Macrophage Exposed to Free Silica or Asbestos Dust in Vitro
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Abstract
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In pneumoconiosis as other chronic interstitial lung diseases, many of the alteration in the lung structure and function are belived to be a consequence of the accumulation and activation of inflammatory cells in the lower respiratory tract.
These
activated cells usually secrete the inflammatory and fibrogenic mediators.
To evaluate the therapeutic effect of colchicine which has the activity to inhibit the secretion of protein in pneumoconiosis, alveolar macrophages were cultured with crocidolite or silica in the presence or absence of colchicine 5㎍/ml At 24 and
48
hours after alveolar macrophage cultured with various stimuli, the released protein and LTB4 (leukotriene B4) was measured. Viability and morphologic changes of alveolar macrophages were observed and protein content in alveolar macrophage lysate
was
quantified.
@ES The results were as follows :
@EN 1. The viability of alveolar macrophages stimulated with crocidolite or silica significantly decreased compared to control At lower dust concentration, crocidolite was more cytotoxic than silica.
2. Colchicine has no effect on the viability of alveolar macrophage.
3. While there was a significant negative correlation between the protein content in lysate and dust concentration, there was a significant positive correlation between the protein content in supernatant and dust concentration.
4. Incubation of alveolar macrophages with dust in the presence of colchicine resulted in significant decrease of protein content in supernatant and cell cell lysate.
5. LTB4 production from alveolar macrohages stimulated with crocidolete or silica significantly increased compared to control Both crocidolite and silica augmented LTB4 production in a concentration-dependent fashion.
6. The production of LTB4 in the group of crocidolite 50㎍/well and silica 50, 500, 1000 and 1500㎍/well treted with colchicine were markedly lower than the groups without colchicine.
The results strongly suggest, that colchicine inhibit the secretion of protein and LTB4 from alveolar macrophage stimulated with crocidolite and silica, even though there was no effect on the viability of alveolar macrophage.
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